Plasmid

Part:BBa_K4730000

Designed by: Sanvi Garg   Group: iGEM23_WVHS-SanDiego   (2023-10-06)


Level 1 MHETase plasmid

This is our level 1 plasmid with the MHETase gene sequence. It contains an inducible Rhamnose promoter and the ErrnB terminator. We are planning on combining it with the PETase plasmid to make our level 2 plamsids that would be able to degrade PET plastics into the harmless byproducts.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1175
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 992
    Illegal SapI.rc site found at 1291


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